P‐17 In vitro activity of posaconazole and other antifungals against Malasseziapachydermatis isolated from dogs
Identifieur interne : 000591 ( France/Analysis ); précédent : 000590; suivant : 000592P‐17 In vitro activity of posaconazole and other antifungals against Malasseziapachydermatis isolated from dogs
Auteurs : P. Bourdeau [France] ; A. M. Marchand [France] ; F. Etoré [France]Source :
- Veterinary Dermatology [ 0959-4493 ] ; 2004-08.
Abstract
The aim of this study was to determine the in vitro antifungal activity of several antifungal drugs (posaconazole, nystatin, miconazole and clotrimazole) against Malassezia pachydermatis with microdilution and agar dilution techniques. Malassezia pachydermatis isolates were obtained from the skin and ears of dogs. Tests on solid media were performed using 25‐well Petri dishes (2 mL/well containing Sabouraud's dextrose agar and diluted antifungal drug) inoculated with 5 μL suspensions of M. pachydermatis. Microtitre broth dilution used 96‐well microtitre plates containing Sabourauds dextrose broth and appropriate dilutions of antifungal drugs, inoculated with 10 μL standard suspensions of M. pachydermatis. Plates were inoculated in duplicate and incubated at 30°C for 5 days and growth assessed. The four antifungal drugs were tested in 10 dilutions (4.0‐0.007 μg/mL for posaconazole, and 32‐‐0.06 μg/mL for clotrimazole, miconazole and nystatin). Results obtained for 83 strains of M. pachydermatis and a control reference strain (CBS 1879) exhibited the same pattern. Results of the MIC between microtitre and agar methodologies showed no significant differences (≤ 2‐fold) across all drugs. For both solid and liquid methods, posaconazole was the most effective antifungal drug of the four tested with MIC90 of 1–2 μg/mL for posaconazole, 16–32 μg/mL for clotrimazole, and ≥ 32 μg/mL for miconazole and nystatin. Funding: Schering‐Plough.
Url:
DOI: 10.1111/j.1365-3164.2004.00414_17.x
Affiliations:
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<hi rend="italic">In vitro</hi> activity of posaconazole and other antifungals against <hi rend="italic">Malassezia</hi><hi rend="italic">pachydermatis</hi> isolated from dogs</title><author><name sortKey="Bourdeau, P" sort="Bourdeau, P" uniqKey="Bourdeau P" first="P." last="Bourdeau">P. Bourdeau</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>National Veterinary School of Nantes, Nantes, France; Schering‐Plough Vétérinaire, Levallois‐Perret</wicri:regionArea><wicri:noRegion>Levallois‐Perret</wicri:noRegion><wicri:noRegion>Levallois‐Perret</wicri:noRegion></affiliation></author><author><name sortKey="Marchand, A M" sort="Marchand, A M" uniqKey="Marchand A" first="A. M." last="Marchand">A. M. Marchand</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>National Veterinary School of Nantes, Nantes, France; Schering‐Plough Vétérinaire, Levallois‐Perret</wicri:regionArea><wicri:noRegion>Levallois‐Perret</wicri:noRegion><wicri:noRegion>Levallois‐Perret</wicri:noRegion></affiliation></author><author><name sortKey="Etore, F" sort="Etore, F" uniqKey="Etore F" first="F." last="Etoré">F. Etoré</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>National Veterinary School of Nantes, Nantes, France; Schering‐Plough Vétérinaire, Levallois‐Perret</wicri:regionArea><wicri:noRegion>Levallois‐Perret</wicri:noRegion><wicri:noRegion>Levallois‐Perret</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Veterinary Dermatology</title><title level="j" type="alt">VETERINARY DERMATOLOGY</title><idno type="ISSN">0959-4493</idno><idno type="eISSN">1365-3164</idno><imprint><biblScope unit="vol">15</biblScope><biblScope unit="page" from="46">46</biblScope><biblScope unit="page" to="46">46</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2004-08">2004-08</date></imprint><idno type="ISSN">0959-4493</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0959-4493</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The aim of this study was to determine the in vitro antifungal activity of several antifungal drugs (posaconazole, nystatin, miconazole and clotrimazole) against Malassezia pachydermatis with microdilution and agar dilution techniques. Malassezia pachydermatis isolates were obtained from the skin and ears of dogs. Tests on solid media were performed using 25‐well Petri dishes (2 mL/well containing Sabouraud's dextrose agar and diluted antifungal drug) inoculated with 5 μL suspensions of M. pachydermatis. Microtitre broth dilution used 96‐well microtitre plates containing Sabourauds dextrose broth and appropriate dilutions of antifungal drugs, inoculated with 10 μL standard suspensions of M. pachydermatis. Plates were inoculated in duplicate and incubated at 30°C for 5 days and growth assessed. The four antifungal drugs were tested in 10 dilutions (4.0‐0.007 μg/mL for posaconazole, and 32‐‐0.06 μg/mL for clotrimazole, miconazole and nystatin). Results obtained for 83 strains of M. pachydermatis and a control reference strain (CBS 1879) exhibited the same pattern. Results of the MIC between microtitre and agar methodologies showed no significant differences (≤ 2‐fold) across all drugs. For both solid and liquid methods, posaconazole was the most effective antifungal drug of the four tested with MIC90 of 1–2 μg/mL for posaconazole, 16–32 μg/mL for clotrimazole, and ≥ 32 μg/mL for miconazole and nystatin. Funding: Schering‐Plough.</div></front></TEI><affiliations><list><country><li>France</li></country></list><tree><country name="France"><noRegion><name sortKey="Bourdeau, P" sort="Bourdeau, P" uniqKey="Bourdeau P" first="P." last="Bourdeau">P. Bourdeau</name></noRegion><name sortKey="Etore, F" sort="Etore, F" uniqKey="Etore F" first="F." last="Etoré">F. Etoré</name><name sortKey="Marchand, A M" sort="Marchand, A M" uniqKey="Marchand A" first="A. M." last="Marchand">A. M. Marchand</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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